Regeneration of Sedum praealtum A.DC (siempreviva) via organogenesis
DOI:
https://doi.org/10.21640/ns.v8i17.637Keywords:
in vitro, indirect organogenesis, regeneration, siempreviva.Abstract
Siempreviva (Sedum praealtum A. DC) belongs to the Crassulaceae family, it has long been used in Mexican ethnobotanical as an anti-inflammatory and analgesic agent in the treatment of toothache, tonsillitis, for eyes diseases, sking and tisuue regeneration eruption, was recently discovered antioxidant compounds havings hepatoprotective and anticancer effects. An efficient protocol for organogenesis regeneration was established for Sedum praealtum from young leaves of nursery plants. Leaf segment that contained the middle portion of the leaf were cultured on Murashige and Skoog medium supplemented with different combintions of 6- Benzylaminopurine (6-BAP) and 2,4-Dichlorophenoxyacetic acid (2,4-D). 90 days after culture, the highest shoot induction was observed on MS medium supplemented with 0.5 mg/L 6-BAP and 0.0 mg/L 2,4-D with 13.0±0.3 shoot per explant. Shoot were individualized and transferred to MS médium supplemented with 2.0 mg/L Indoleacetic acid (IAA) for rooting.Downloads
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