Effects of Leucaena leucocephala intake on testicular characteristics in pubescent ram (Ovis aries)
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Sertoli reproduction

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Núñez Martínez, G., Sánchez Santillán, P., Aguilar Marcelino, L., Martínez Maya, J. J., Millán Orozco, J., & Bottini Luzardo, M. B. (2022). Effects of Leucaena leucocephala intake on testicular characteristics in pubescent ram (Ovis aries). Nova Scientia, 14(29). https://doi.org/10.21640/ns.v14i29.3109


The leaves of the tropical tree Leucaena leucocephala are often used as a livestock feed input. However, they contain mimosine, an antinutritional factor that can negatively affect animal physical parameters. An evaluation was done of testicular characteristics in pubescent ram that had consumed fresh L. leucocephala leaves. Experimental animals were 20, 5-month-old male Creole ram (average weight = 24.4 ± 3.21 kg) housed in individual pens. During the 120 d experimental period 10 males were fed a 0 % Leucaena diet (control) and another 10 were fed a diet including 25 % fresh L. leucocephala (Leucaena). Real leucocephala intake was determined by the difference between the quantity that was offered, and the quantity was rejected. For the determination of testosterone and mimosine in blood, an ELISA kit and HPLC were used respectively. Scrotal circumference measurements were taken, and testicular volume was estimated. A histological study was carried out; to determine the integrity of the testicular parenchyma testicular. The 40 % of male’s intake L. leucocephala presented interstitial edema and tubular atrophy in the testicular parenchyma of pubescent ram. Scrotal circumference and testicular volume were not affected; however, edema and atrophy of the seminiferous tubules were found in animals that intake Leucaena leucocephala

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AFRC. (1995). AFRC. Energy and Protein Requirements of Ruminants. An Advisory Manual Prepared by the AFRC Technical Committee on Responses to Nutrients. University Press (Eds.)(1995). Cambridge U.K. CAB-INTERN. Edited by B. C. G. Alderman. Wallingford: University Press, Cambridge.

Almeida A. P., Kommers M., Nogueira A., Júnior, L. G. B., Prado Marques, B. M. F., y Lemos, R. (2006). Avaliação Do Efeito Tóxico de Leucaena Leucocephala (Leg. Mimosoideae) Em Ovinos. Pesquisa Veterinária Brasileira, 26(3),190–94. https://doi.org/10.1590/s0100-736x2006000300011.

Alvarado P., y Grajales, H. (1989). Protocolo Toma de Muestra de Sangre En La Especie Ovina. Vol. 53. http://medicinaveterinariaydezootecnia.bogota.unal.edu.co/fileadmin/FVMZ/Servicios/bioetica/Pro_autorizados/003_Protocolo_muestreo_sanguineo_ovinos-CIDTEO.pdf

Bacab-Pérez, H., y Solorio-Sánchez, F. (2011). Forage Offer and Intake and Milk Production in Dual Purpose Cattle Managed under Silvopastoral Systems in Tepalcatepec, Michoacan. Tropical and Subtropical Agroecosystems, 13(3), 271–78. http://dx.doi.org/urn:ISSN:1870-0462-tsaes.v1i3.1367

Bairagi, A., Sarkar Ghosh, K., Sen, S. K., y Ray, A. K. (2004). Evaluation of the Nutritive Value of Leucaena leucocephala Leaf Meal , Inoculated with Fish Intestinal Bacteria Bacillus Subtilis and Bacillus Circulans in Formulated Diets for Rohu , Labeo Rohita ( Hamilton ) Fingerlings. Aquaculture Research 35, 436–46. http://doi.org/10.1111/j.1365-2109.2004.01028.x

Barros-Rodríguez, M., Solorio-Sánchez, F., Ku-Vera, J., Ayala-Burgos, A., Sandoval-Castro, C., y Solís-Pérez, G. (2012). Productive Performance and Urinary Excretion of Mimosine Metabolites by Hair Sheep Grazing in a Silvopastoral System with High Densities of Leucaena Leucocephala. Tropical Animal Health and Production, 44(8), 1873–78. http://doi.org/ 10.1007/s11250-012-0150-0.

Berndtson, W. (1977). Methods for Quantifying Mammalian Spermatogenesis: A Review. Journal of Animal Science 44, 818–33.

Blache, D., Adam, C. L., y Martin, G. B. (2002). The Mature Male Sheep: A Model to Study the Effects of Nutrition on the Reproductive Axis. Reproduction (Cambridge, England) Supplement, 59, 219–33.

Bottini-Luzardo M., Aguilar-Perez C., Centurion-Castro F., Solorio-Sanchez, F., Guillermo L., Muñoz-Rodriguez D., Salas-Araujo Y., y Ku-Vera, J. (2018). Consumo de mimosina en el desarrollo de glándulas endometriales de vacas en postparto temprano. Agrociencias 52(6), 817-826. https://agrociencia-colpos.mx/index.php/agrociencia/article/view/1706/1706.

Brito L., Albert F. C., Barth, D., Rawlings, N., Wilde, R., Crews, D., Mir, P., y Kastelic, J. (2007). Effect of Improved Nutrition during Calfhood on Serum Metabolic Hormones, Gonadotropins, and Testosterone Concentrations, and on Testicular Development in Bulls. Domestic Animal Endocrinology, 33(4), 460–69. http://doi.org//10.1016/j.domaniend.2006.09.004.

Cabrejos Pita, G., Lisigurski Teitelman, M., Delgado Chumpitaz, D., Matos Villegas, G., León Alvare,z L.A., & Cabello Morales, E. (2002). Determinación Del Volumen Testicular y Longitud Del Pene En Escolares de 5 Años a 9 Años de Edad En El Distrito de San Martin de Porres En Lima Metropolitana. Revista Medica Herediana 13(3), 79–84. http://doi.org// 10.20453/rmh.v13i3.694.

Chacón, J., Harvey Lozano, L., Orozco, C., y Ariosto Ardila, S. (2019). Características de La Pubertad En Corderos de Pelo y Sus Cruces En Colombia En Condiciones de Baja Altitud. Revista MVZ Cordoba 24(1), 7097–7103. 10.21897/rmvz.1227.

Chanchay, N., y Poosaran, N. (2009). The Reduction of Mimosine and Tannin Contents in Leaves of Leucaena Leucocephala. Asian Journal of Food and Agro-Industry, Special Issue, S137–44.

Galaviz-Rodríguez, J. R., Ramírez-Bribiesca, J. E., Vargas-López, S., Zaragoza-Ramírez, J. L., Guerrero-Rodríguez, J. D., Mellado-Bosque M., y Ramírez, R. G. (2014). Effect of Three Production Systems of Central Mexico on Growth Performance of Five Lamb Genotypes. Journal of Animal and Plant Sciences 24(5), 1303–8.

García, M., G., Gonzáles, W., Medina, R., y Cova, O. (2008). Caracterización de Diez Cultivares Forrajeros de Leucaena leucephala Basada en la Composición Química y La Degradabilidad Ruminal. Revista MVZ Cordoba, 13(2), 1294–1303.

Gutteridge R. C., y Shelton H. M. (1998). Forage Tree Legumes in Tropical Agriculture Titles of Related Interest from CAB International: Grassland Dynamics: An Ecosystem Simulation Model. C. Jarvis and B.F. Pain 1(2). https://betuco.be/coverfodder/Forage%20Tree%20Legumes%20in%20Tropical%20Agriculture%20FAO.pdf

Hammond A. C. (1995). Leucaena Toxicosis and Its Control in Ruminants. Journal of Animal Science, 73, 1487–92. https://doi.org/10.2527/1995.7351487x.

Kakengi A. M., Shem M. N., Mtengeti E. P., y Otsyina, R. (2001). Leucaena leucocephala Leaf Meal as Supplement to Diet of Grazing Dairy Cattle in Semiarid Western Tanzania. Agroforestery Systems 52, 73–82.

De la Vega A., Morales P., Zimerman, M., y Wilde, O. (2006). Variación Anual de La Circunferencia Escrotal En Caprinos Criollos Serranos. Archivos de Zootecnia 55(209), 113–16.

Medina, R., y Sánchez, A. (2006). Efecto de La Suplementación Con Follaje de Leucaena leucocephala Sobre La Ganancia de Peso de Ovinos Desparasitados y No Desparasitados Contra Estrongílidos Digestivos. Zootecnia Tropical, 24(1), 55–68.

Moghaddam, G., Pourseif, M. M., Asadpour, R., Abass Rafat S., y Jafari-Jozani, R. (2012). Relationship between Levels of Peripheral Blood Testosterone, Sexual Behavior, Scrotal Circumference and Seminal Parameters in Crossbred Rams. Acta Scientiae Veterinariae, 40(3): 1–8.

Mohammadabadi, T., y Jolazadeh, A. (2017). Replacement of Alfalfa Hay (Medicago sativa L.) with Subabul on Digestion Activity of Rumen Microoganisms. Tropical Animal Health and Production 49, 1309–16. 10.1007/s11250-017-1330-8.

Nieves, D., Silva, L., Terán, O., y González, C. (2002). Niveles Crecientes de Leucaena leucocephala En Dietas Para Conejos de Engorde. Revista Científica, XII(2), 419–21.

Orden, E., Abdulrazak, S., Cruz, E. M., Ichinohe, T., y Fujihara, T. (2000). Leucaena leucocephala and Gliricidia sepium Supplementation in Sheep Fed with Ammonia Treated Rice Straw: Effects on Intake, Digestibility, Microbial Protein Yield and Live-Weight Changes. Asian-Australasian Journal of Animal Science 13(12), 1659–66.

Rahman, M., Wahab, M. A., Ahmed, A., y Tyeb, A. (1988). The effects of feeding Leucaena leucocephala to Tilapia niolitica. Leuc. Res. Repts 9, 35-36.

Santos Ricalde R., y Abreu Sierra J. (1995). Evaluación Nutricia de La Leucaena Leucocephala y Del Brossimun Alicastrum y Su Empleo En Alimentación de Cerdos. Veterinaria México 26(1), 51–57.

Shelton, H. M., y Brewbaker J. L. (1994). Leucaena leucocephala -the Most Widely Used Forage Tree Legume Introduction Botanical Description and Genetic Variation Uses Climate and Soil Adaptation Establishment Toxicity Pests and Diseases Conclusions References. Agroforestería Para La Producción Animal En Latinoamérica 9(3), 367–94.

Suharti, S., Shila Kurnia, F.X., Pambudi, B., y Wiryawan, K. G. (2018). Fate of Mimosine, Concentration of Blood Metabolites and Thyroid Hormones of Sheep Fed with Leucaena and Glyricidia Leaf Meal. Pakistan Journal of Nutrition 17(6), 268–73. 10.3923/pjn.2018.268.273.

Tapia Argûelles, L., López Rodríguez, J. L., Bueno Ortega, J., y Riva Rodríguez, R. (2000). Hojas de Leucaena leucocephala En La Alimentación de Patos En Crecimiento. Revista de Producción Animal 12(1), 17.

Vázquez Martínez, I., Jaramillo Villanueva, J., Bustamante González, A., Vargas López, S., Calderón Sánchez, F., Torres Hernández, G., y Pittroff, W. (2018). Estructura y Tipología de Las Unidades de Producción Ovinas En El Centro de México. Agricultura Sociedad y Desarrollo 15(1), 85. 10.22231/asyd.v15i1.750.

Woldemeskel, M., Tegegne, A., Umunna, N., Kaitho, R.J., y Tamminga, S. (2001). Effects of Leucaena pallida and Sesbania sesban Supplementation on Testicular Histology of Tropical Sheep and Goats. Animal Reproduction Science 67(3–4), 253–65. 10.1016/S0378-4320(01)00131-2.

Wu, C., Min Yuan, H., Jia, G., Sheng Wang, S., y Qun Wu, X. (2012). Determination of Mimosine and 2,3-Dihydroxypyridine in Leucaena Leucocephala by Reversed Phase High-Performance Liquid Chromatography. Applied Mechanics and Materials 140, 296–301. 10.4028/www.scientific.net/AMM.140.296.

Yakubu, M. T. (2012). Effect of a 60-Day Oral Gavage of a Crude Alkaloid Extract from Chromolaena Odorata Leaves on Hormonal and Spermatogenic Indices of Male Rats. Journal of Andrology 33(6), 1199–1207. 10.2164/jandrol.111.016287.

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