In vitro effect of an immunostimulant bacteria on the morphology of bovine monocytes
DOI:
https://doi.org/10.21640/ns.v7i15.263Keywords:
cattle, monocytes, lactobacilli, cultureAbstract
To assess changes in cell morphology as sign of activation, peripheral blood monocytes from four adult cattle were obtained and cultured in vitro in the presence or not of Lactobacillus casei. At 48 and 72 hours after exposure to the bacteria, cells were stained and then, under the optical microscope, the circumference of the plasma membrane (CPM) and the maximum length of the cytoplasm (MLC) was determined. Data obtained (+ SEM) of the CPM and MLC (n=20/bovine) for control cells (T) and treated with L. casei (LC), were for CPM: 41.54 (+ 2.08) µm for T and 61.53 (+ 2.30) µm for LC at 48 hours (P <0.05) and 35.80 (+ 1.49) µm for T and 68.90 (+ 8.8) µm for LC at 72 hours (P <0.05). The average values of MLC obtained were 13.08 (+ 1.05) µm for T and 19.34 (+ 0.86) µm for LC at 48 hours (P <0.05) and 11.46 (+ 0.67) µm for T and 21.55 pp: 01 - 10 - 3 - (+ 3.3) µm for LC at 72 hours (P <0.05). The results obtained suggest that L. casei activated monocytes to be transformed into macrophages, which are key cells in the immune response.Downloads
References
Adams D.O. (1979). Macrophages. En: Method and Enzymology LVIII Academic Press Inc. pp 494-506.
Bautista-Garfias C.R., Ixta-Rodríguez O., Martínez-Gómez F., López M.G., Aguilar-B.R. (2001). Effect of viable or dead Lactobacillus casei organisms administered to mice on resistance against Trichinella spiralis infection. Parasite, 8: S226-S228.
Bautista-Garfias C.R., Gómez M.B, Aguilar B.R., Ixta O., Martínez F., Mosqueda J. (2005). The treatment of mice with Lactobacillus casei induces protection against Babesia microti infection. Parasitol. Res. 97:472-477.
Bautista-Garfias C.R., Mosqueda-Gualito J.J.(2005). Papel de los receptores Toll-like en la inmunidad innata y su implicación en medicina veterinaria. Vet. Méx., 36:453-468.
Bautista-Garfias CR, Castañeda R, Álvarez JA, Rojas C, Figueroa JV, Rodríguez A. (2012). La vacunación simultánea de bovinos con Lactobacillus casei y la vacuna bivalente contra babesiosis bovina genera una mayor protección contra Babesia bovis y B. bigemina transmitidas por garrapatas en condiciones extremas de campo. Vet. Méx., 43:189-200.
Birminham JR , Jesk EL (1980) The isolation, long-term cultivation and characterization of bovine peripheral blood monocytes. Immunology 41:807-814.
Jungi TW, Thony M, Brcic M, Adler B, Pauli U, Peterhans E. (1996) Induction of nitric synthase in bovine mononuclear phagocytes is differentiation stage-dependent. Immunobiology 195:385-400.
Kim YG, Ohta T, Takahashi T, Kushiro A, Nomoto K, Yokokura T, Okada N, Danbara H. (2006). Probiotic Lactobacillus casei activates innate immunity via NF-kB and p38 MAP kinase signaling pathways. Micr. Infect. 8:994-1005.
Kwissa M., Amara R.R., Robinson H.L., Moss B., Alkan S., Jabbar A., Villinger F., Pulendran B. (2007). Adjuvanting a DNA vaccine with a TLR9 ligand plus Flt3 ligand results in enhanced cellular immunity against the simian immunodeficiency virus. J. Exp. Med.; 204:2733–2746.
Maldonado Galdeano C, Perdigon G. (2006). The probiotic bacterium Lactobacillus casei induces activation of the gut mucosal immune system through innate immunity. Clin. Vacc. Immunol. 13:19-226.
Olivares S.(1994). Paquete de diseños experimentales FAUNL, Version 2.5. Facultad de Agronomía, UANL, Marín, N.L., México
Pelletier C, Bouley C, Cayuela C, Bouttier S, Bourliux P, Bellon-Fontaine MN (1997). Cell surface characteristics of Lactobacillus casei subsp. casei, Lactobacillus paracasei subsp. paracasei, and Lactobacillus rhamnosus strains. Appl. Environ. Microbiol. 63:1725-1731.
Plantinga TS, van Maren WWC, van Bergenhenegouwen MH, Nierkens S, de Jong DJ, Joosten LAB, van´t Land B, Garssen J, Adema GJ, Netea MG. (2011). Differential Toll-like receptor recognition and induction of cytokine profile by Bifidobacterium breve and Lactobacillus strains of probiotics. Clin Vacc Immunol 18:621-628.
Ross J.A., Auger M.J. (2002) The biology of the macrophage. En: Burke B., Lewis C.E. (editors). The macrophage. 2nd Ed. Oxford University Press, New York. pp. 1-72.
Saldarriaga OA, Velásquez JI, Ossa JE, Rugeles MT. (2003). Standarization of bovine macrophage monolayers and isolation and culture of trypanosomes. Mem. Inst. Oswaldo Cruz 98:269-271.
Vega C. A, Buening G M, Rodríguez S D. Carson C A. McLaughlin K. (1985). Cryopreservation of Babesia bigemina for in vitro cultivation. Am.J.Vet.Res.; 46:421-423.
Werling D, Hope JC, Howard CJ, Jungi TW (2004).Differential production of cytokines, reactive oxygen and nitrogen by bovine macrophages and dendritic cells stimulated with Toll-like receptor agonists. Immunology 111:41-52.
Wille-Reece U., Flynn B.J., Loré K., Koup R.A., Miles A.P., Saul A., Kedl R.M., Mattapallil J.J., Weiss W.R., Roederer M., Seder R.A. (2006). Toll-like receptor agonists influence the magnitude and quality of memory T cell responses after prime-boost immunization in nonhuman primates. J Exp Med, 203:1249–1258.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2015 Nova Scientia
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Conditions for the freedom of publication: the journal, due to its scientific nature, must not have political or institutional undertones to groups that are foreign to the original objective of the same, or its mission, so that there is no censorship derived from the rigorous ruling process.
Due to this, the contents of the articles will be the responsibility of the authors, and once published, the considerations made to the same will be sent to the authors so that they resolve any possible controversies regarding their work.
The complete or partial reproduction of the work is authorized as long as the source is cited.
