Bioprospecting of haloalkaliphilic microorganisms isolated from a dried-out maar in the volcano “Hoya Rincón de Parangueo”

Authors

  • Elizabeth Selene Gómez-Acata Instituto Tecnológico de Celaya, Departamento de Ingeniería Ambiental https://orcid.org/0000-0003-0738-0483
  • Laura Mariana Ayala-Gámez Centro de Investigación y de Estudios Avanzados del IPN
  • Raúl García-Covarrubias Centro de Investigación y de Estudios Avanzados del IPN
  • Martha Yvette Torres-Núñez Centro de Investigación y de Estudios Avanzados del IPN
  • Liliana Vargas-López Centro de Investigación y de Estudios Avanzados del IPN
  • Susana Hernández-Camargo Centro de Investigación y de Estudios Avanzados del IPN
  • Itzel Guadalupe Becerril-Rojas Centro de Investigación y de Estudios Avanzados del IPN
  • Marcos León-Domínguez Centro de Investigación y de Estudios Avanzados del IPN
  • Lizbeth Zurita-García Centro de Investigación y de Estudios Avanzados del IPN
  • Yendi E. Navarro-Noya Universidad Autónoma de Tlaxcala https://orcid.org/0000-0002-7191-7815
  • Eloy Conde-Barajas Instituto Tecnológico de Celaya, Departamento de Ingeniería Ambiental https://orcid.org/0000-0002-6098-860X
  • Luc Dendooven Centro de Investigación y de Estudios Avanzados del IPN https://orcid.org/0000-0002-4148-2283

DOI:

https://doi.org/10.21640/ns.v13i26.2553

Keywords:

bioprospection, siderophore, halophile, lipases, Nesterenkonia, hypersaline environments, extracellular enzymes, Actinobacteria, Firmicutes, Proteobacteria, bacterial genera

Abstract

Halophiles can be found in hypersaline environments and they can synthesize products of industrial interest.

Method: In this work, 37 bacterial strains were isolated from the sediment of a dried-out maar of the “Hoya Rincón de Parangueo” (Mexico) on different culture media with 20% NaCl and pH 9, while potential industrial extracellular enzymes were determined.

Results: Eight genera Salsuginibacillus, Alkalibacillus, Bacillus, Staphylococcus, Oceanobacillus, Halomonas and Nesterenkonia besides 15 distinct species belonging to Actinobacteria, Firmicutes and Proteobacteria were identified. The L371, LTA7 and SU3 isolates produced proteases, M20, L5, L7, L8, Y1, Y2, M3, M9 and RA lipases, LTA4 and SU3 amylases, while LTA7, Y2, M5, M9 and RA caseinases with enzymatic activity at pH 9. Production of siderophore was found in 17 isolates. Discussion or Conclusion: It is reported for the first experimental evidence of siderophores production by N. alba, Alkalibacillus and Oceanobacillus at 6% NaCl (w/v). Isolates RE, RG1, RG2, RH1, RA and I11 showed 2.8% to 4.1% genetic distance with Nesterenkonia species and might represent new species.

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2021-05-14

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Gómez-Acata, E. S., Ayala-Gámez, L. M., García-Covarrubias, R., Torres-Núñez, M. Y., Vargas-López, L., Hernández-Camargo, S., … Dendooven, L. (2021). Bioprospecting of haloalkaliphilic microorganisms isolated from a dried-out maar in the volcano “Hoya Rincón de Parangueo”. Nova Scientia, 13(26). https://doi.org/10.21640/ns.v13i26.2553

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