Polyploidy in wild blackberries (Rubus spp L.)
DOI:
https://doi.org/10.21640/ns.v10i21.1385Keywords:
crushed roots, chromosomes, phytogenetic resources, morphological variability, blackberries, genetic improvementAbstract
Number of chromosomes is important for evolutionary studies, phylogenetic, genetic and taxonomic improvement. Due the importance of chromosomes, this research was conducted in order to determine polyploidy on blackberries Rubus adenotrichos Schltdl, R. cymosus Rydb, R. humistratus Steud, R. palmeri Rydb, R. pringlei Rydby, R sapidus Schltdl; Tupi, Kiowa, Tupí x R. sapidus and Kiowa x R. sapidus. Chromosome counting was performed on somatic cells by crushing root tips. Chromosomes were observed and counted in metaphase for each one of specie, polyploidy was determined starting from 1x = 7. Morphological characteristics of each one of specie were based on qualitative descriptors of UPOV 2006. Blackberry showed variability in the number of chromosomal games: Rubus adenotrichos (2n = 2x = 14), R. cymosus (2n = 5x = 35), R. humistratus (2n = 2x = 14), R. palmeri (2n = 4x = 28), R. pringlei (2n = 3x = 21), R. sapidus (2n = 3x = 21); Tupi (2n = 2x = 21), Kiowa (2n = 2x = 28), Tupi x R. sapidus (2n = 2x = 21) and Kiowa x R. sapidus (2n = 2x = 28). Each one of the species presented particular characteristics that characterize them, however, it was not posible to determinate the morphological characteristics associate or define the poly-ploidy in the species analyzed.Downloads
References
Acosta-Montoya, Óscar, Fabrice Vaillant, Sonia Cozzano, Mertz, Christian Perez, Ana M. Pérez y Marco V. Castro. (2010). Phenolic content and antioxidant capacity of tropical Highland blackberry (Rubus adenotrichus Schltdl) during three edible maturity stages. Food Chemistry 4 (42):1497-1501.
Bennett, Mike D., Parmjit Bahandol, y Ilia J. Leitch. (2000). Nuclear DNA Amounts in angiosperms and their modern uses 807 new estimates; Annals of Botany 86:859-909.
Bennett, Mike D., y Ilia J. Leitch. (2011). Nuclear DNA amounts in angiosperms: targets, treds and tomorrow. Annals of Botany 107 (3): 467-590.
Fawcett, Jeffrey A., Steve Maere, y Yves Van De Peer. (2009) Plants with doublée genomes migth have had a better chance to survive the Cretaceous-Tertiaruy extinction event. Proceedings of the National Academy of Sciences of the United State of America 106 (14):5737-5742.
Galbraith, David W., Kristi R. Harkins, Joyce M. Mardox, Nicola M. Ayres, Dharam P. Sharma y Ebrahim Firoozabady. (1983). Rapid flow cytometric analysis of the cell cycle in intact plant tissue. Science 220 (4601):1049-1051.
Gianoli, Ernesto. (2004). Plasticidad Fenotípica Adaptativa en Plantas. En: Fisiología Ecológica en Plantas: Mecanismos y Respuestas al Estrés en los Ecosistemas; M. Cabrera. (ed). Departamento de Botánica. Universidad de Concepción, Chile. pp: 13-25.
Grandont, Laurie, Eric Jeczewski, y Andrew H. Lloyd. (2013). Meiosis and its deviations in polyploid plants; Cytogenetic and Genome Research 140:171-184.
Heslop-Harrison, Pat, y Trude Schwarzacher. (2011). Organization of the plant genome in chromosomes; Plant Journal 66:18-33.
Human, Zósimo. (1995). Tecnicas citologicas para determiner el numero cromosomico y la fertilidad de las papas. Centro Internacional de la papa. Lima. Perú. 19 p.
Jeczecwski Eric, y Karine Alix. (2004). From diploids to allopoplyploids the emergence of efficient pairing control genes in plants. Critical Reviews in Plant Sciences 23:21-45.
Levin, Donald A. (2002). The Role of Chromosomal Change in Plant Evolution. Oxford Series in Ecology and Evolution; Oxford University Press. 231 p.
Lim, K. Yoong, Ilia J. Leitch, y Andrew R. Leitch. (1998) Genomic characterisation and the detection of raspberry chromatin in poliploid Rubus. Theoretical and Applied Genetics 97 (7):1027-1033.
Lobo, Mario. (2006). Recursos genéticos y mejoramiento de frutales andinos: Una visión conceptual; Revista Corpoica-Ciencia y Tecnologia Agropecuaria 7 (2):40-54.
Marcela-Delgado, Lina; Marcela Uribe-Lastra, Marta Leonor Angel Marulanda. (2010). Estandarización de la técnica citogenética “Squash” para conteo de cromosomas mitóticos en Rubus glaucus Benth. Scientia et Technica 46 (17):74-79.
Martínez-Cruz, Nieves del Socorro, Katiushka Arévalo-Niño, María Julia Verde-Star, Catalina Rivas-Morales, Azucena Oranday-Cardenas, María Adriana Núñez-González y María Eufemia Morales-Rubio. (2011). Antocianinas y actividad anti radicales libres de Rubus adenotrichus Schltdl (Zarzamora). Revista Mexicana de Ciencias Farmacéuticas 4(42):66-71.
Mastersoon, Jane. (1994). Stomatal size in fossil plants: evidence for polyploidy in majority of angiosperms. Science 264 (5157):421-423.
Meng, Rengong y Chad Finn. (1999). Using Flow cytometry to determine ploidy level in Rubus. Acta Horticulturae 505:223-227.
Meng, Rengong y Chad Finn. (2002). Determining ploidy level and nuclear DNA content in Rubus by cytometry. Journal of the American Society for Horticultural Science 127 (5):767-775.
Mercier, Raphaël, Christine Mézard, Eric Jenczewski, Nicolas Macaisne y Mathilde Grelon. (2015). The molecular biology of Meiosis in plants. Annual Rewiew Plant Biology 66:297-327.
Mertz, Christian, Veronique Cheynier, Ziya Gunata, y Pierre Brat. (2007). Analysis of phenolic compounds in two blackberry species (Rubus glaucus and Rubus adenotrichus Schltdl) by High-Performance Liquid Chromatography with diode array detection and electrospray ion trap mass spectrometry. Journal Agriculture Food 55 (21):8616-8624.
Moreno, M; Villarreal, D; Lagos, T. C; Ordoñez, H; y Hernando Criollo. (2011). Caracterización “ In-situ” de genotipos silvestres y cultivados de mora Rubus spp en el municipio de pasto; Revista de Ciencias Agricolas 2 (28):109-128.
Nathewet, Preeda; Tomohiro Yanagi, Yoshikane Iwatsubo, Kazuyoshi Sone, Takehiro Takamura y N. Okuda. (2009). Improvement of staining method for observation of mitotic chromosomes in octoploid strawberry plants; Scientia Horticulturae 120:431-435.
Otto, Sara P., y Jeanette Whitton. (2000). Polyploid incidence and evolution. Annual Reviews of Genetics 34:401-437.
Poehlman, John Milton y David Allen. (2002). Mejoramiento Genético de las Cosechas. Editorial Limusa. México. 506 p.
Poehlman, John Milton y David Allen. (2005). Citotipos en Agave angustifolia Haw determinados por citometría de flujo y análisis en sus cariotipos; Revista Internacional de Contaminación Ambiental. 1(21):49-54.
Proost, Sebastian, Pattyn Pedro, Gerats Tom y Yves Van Der Peer. (2011). Journey through the past: 150 million years of plant genome evolution. Plant Journal 66 (1):58-65.
Ramsey, Justin y Douglas W. Schemskse. (1998). Pathways, mechanisms, and rates of polyploid formation in flowering plants; Annual Review of Ecology and Systematics. 29:467-501.
Ramsey, Justin y Douglas W. Schemskse. (2002). Neo polyploidy in flowering plants plants. Annual Rewiews in Ecology and Sistematics. 33:589-639.
Rieseberg, Loren H y John H. Willis. (2007). Plant speciation; Science 5840 (317):910-914.
Soltis, Douglas E. Y J. Gordon Burleigh. (2009). Surviving the K-T mass extinction: new perspectives of polyploidization in angiosperms. Proceeding of the National Academy of Sciences of the United States of America 106 (7):5456-5456.
Thompson, Maxine M. (1995). Chromosome numbers of Rubus cultivars at the National Clonal Germplasm Repository. HortScience 30 (7):1453-1456.
Thompson, Maxine M. (1997). Survey of chromosome number in Rubus (Rosaceae: Rosoideae); Annals of the Missouri Botanical Garden 84(1):129-165.
Union Internacional Proteccion de las Obtenciones Vegetales (UPOV). (2006). Rubus subgenus Eubatus sect. Moriferi & Ursini e híbridos (www.upov.int), consultada, octubre 2015.
Zamorano M. Adriana, Ana Cruz Morillo, Yacenia Morillo, Herney Vasquez y Jaime E. Muñoz. (2007). Caracterización morfológica de mora en los departamentos del Valle del Cauca, Cauca y Nariño de Colombia. Acta Agronómica 56 (2):51-60.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2018 Nova Scientia
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Conditions for the freedom of publication: the journal, due to its scientific nature, must not have political or institutional undertones to groups that are foreign to the original objective of the same, or its mission, so that there is no censorship derived from the rigorous ruling process.
Due to this, the contents of the articles will be the responsibility of the authors, and once published, the considerations made to the same will be sent to the authors so that they resolve any possible controversies regarding their work.
The complete or partial reproduction of the work is authorized as long as the source is cited.
